What is the 2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride?

2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride is White crystalline powder, while it's Molecular Formula is C4H12ClNO3. White crystalline powder TRIS hydrochloride is a high quality buffer component used in SDS-PAGE. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. It is also used in the robust generation of transgenic mice.

What is the 2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride?

2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride is White crystalline powder, while it's Molecular Formula is C4H12ClNO3. White crystalline powder TRIS hydrochloride is a high quality buffer component used in SDS-PAGE. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. It is also used in the robust generation of transgenic mice.

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Q: What is the CAS number for 2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride?

The CAS number of 2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride is 1185-53-1.

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2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride

CAS No.: 1185-53-1

product details:

Purity: 99%

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Molecular Formula: C4H12ClNO3
Molecular Weight: 157.597
Appearance/Colour: White crystalline powder
Vapor Pressure: 0Pa at 20℃
Melting Point: 150-152 ºC
Boiling Point: 357 ºC at 760 mmHg
PKA: 8.1(at 25℃)
Flash Point: 169.7 ºC
PSA： 86.71000
Density: 1.05g/mLat 20°C
LogP: -0.83690

2-Amino-2-(hydroxymethyl)-1,3-propanediol hydrochloride(Cas 1185-53-1) Usage

Preparation	The preparation of TRIS hydrochloride is as follows:After reacting 2-amino-1,3-propanediol (2.50 g, 27.44 mmol) and formic acid (1.26 g, 27.44 mmol) in 50 ml of water at room temperature for 3 hours, water was distilled off under reduced pressure, A liquid was obtained. By washing the obtained liquid,An ammonium formate salt (3.76 g, 27.44 mmol) was obtained as a colorless transparent liquid.
Purification Methods	Crystallise the salt from 50% EtOH, then from 70% EtOH. TRIS-hydrochloride is also available commercially in a highly pure state. Otherwise, recrystallise it from 50% EtOH, then 70% EtOH, and dry it below 40o to avoid risk of decomposition. [Beilstein 4 H 304.]

Uses and Mechanism of Action	2-Amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride (Tris-HCl) is primarily used as a buffering agent in various fields, including laboratory chemicals, pH regulators, water treatment products, and chemicals for health services and scientific research. Tris-HCl is utilized to maintain a stable pH environment, particularly in applications where an acidic pH is required. Tris-HCl can influence the corrosion behavior of certain metals, such as magnesium and zinc alloys, in various environments by affecting pH levels and the formation of corrosion products.
Production Methods	2-Amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride (Tris-HCl) is typically synthesized by combining tris(hydroxymethyl)aminomethane with hydrochloric acid.
Application	TRIS hydrochloride is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.
General Description	Trizma? hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.